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Wanna watch Bollywood’s biggest hit movie go here download file search,.Reduced expression of c-fos in the rat thalamus after prolonged morphine treatment: effects of naloxone and neonatal handling.
Our previous studies show that chronic morphine (5 mg/kg daily s.c. for 7 days) decreases the number of c-Fos-like immunoreactive cells in the thalamus of adult rats, the decrease being significantly larger in rats submitted to a form of neonatal handling (daily 15 min handling) than in unhandled rats. The present report analyzes the time course of the decrease in c-Fos immunolabeling in the ventrobasal thalamic complex of adult rats submitted or not to chronic morphine treatment or neonatal handling. It is shown that neonatal handling significantly reduced the number of c-Fos immunoreactive cells in the ventrobasal complex of the thalamus of chronically treated rats and in this group, the decrease was maximal after 3 weeks of treatment. In contrast, in unhandled rats treated with morphine for 7 days and in unhandled untreated rats, a decrease in c-Fos immunostaining was detected within 48 h. The decrease was large in the lateral thalamus and moderate or small in the ventrobasal complex of the thalamus. Naloxone (1 mg/kg) given 30 min before chronic morphine treatment (7 days) restored in the ventrobasal complex the number of c-Fos-like immunoreactive cells to that seen in saline-treated rats. The data show that c-Fos is significantly decreased in the ventrobasal thalamus after prolonged morphine treatment, that morphine treatment reduces the number of c-Fos immunoreactive neurons only in rats that have been submitted to neonatal handling and that naloxone corrects the decrease in c-Fos immunoreactivity in ventrobasal thalamic cells of chronically morphine-treated rats.Purification and characterization of malto-dextrinsucrase from Alvarado sludge.
In this work the malto-dextrin sucrase enzyme from Alvarado sludge was isolated. Enzymatic activity was estimated at 37 degrees C and at pH 6.0, in the absence of 2-mercaptoethanol, with 18% of malto-dextrins produced. The sucrase was isolated from the Al
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