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References

Category:CAD software for WindowsStructural determinants of Asp-N-glycosidase/asparaginase single-chain antibody for targeted enzyme delivery into cancer cells.
A single-chain antibody against Asp-N-glycosidase/asparaginase (ASNase) of Escherichia coli (EcASNase) was designed and constructed to enhance the cytotoxicity of EcASNase against cancer cells by conjugating EcASNase to the antibody. In order to evaluate the feasibility of this designed single-chain antibody (ScFv) to EcASNase for targeted enzyme delivery, the characterization of the conjugation of EcASNase to the ScFv was carried out. The conjugation was confirmed by SDS-PAGE, ELISA, and haemolytic activity tests. The circular dichroism spectra analysis indicated that the secondary structure of the ScFv was slightly affected by the enzyme conjugation. The haemolytic activity was much lower for the enzyme-conjugated ScFv than for the EcASNase. However, the hybrid EcASNase-ScFv revealed more cytotoxicity than EcASNase in two different cancer cell lines, MIA PaCa-2 and PC-3. These results suggest that the designed ScFv may be able to function as a targeting group for enzyme delivery into cancer cells.The Minnesota Timberwolves today announced their schedule for next season, as well as various special events at Target Center.

RELATED STORIES

Season Ticket Holders Will Get Discount on Certain Seats

Timberwolves Announce ‚Team Vision Walk‘, ‚Gameday Spectacular‘

Ben Gordon, Charlie Villanueva and other athletes participate in ‚At the Buzzer‘

Fans will also have the opportunity to purchase one-day tickets for the team’s home opener next season by visiting TargetCenter.com.

The Timberwolves will unveil their new look at a private event today in Minneapolis, followed by a public unveiling at Team Vision Walk at 7 p.m. July 17. Fans will have a unique opportunity to see the new look team as well as meet the players for the first time.

All proceeds from the July 17 event will go to the Timberwolves Dream Fund, created by the owners and players to provide financial support to Minnesota families.

Special bonus pricing for all Timberwolves regular-season games
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