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To determine the effects of estrogen-progestin regimens on postheparin lipase activities, plasma concentrations of insulin, glucose, triglyceride, and cholesterol, and adipose tissue lipoprotein lipase (LPL) activity, eight postmenopausal women were randomly assigned in a double-blind crossover study to receive a placebo or one of two different estrogen-progestin regimens for 10 days. During the last 2 days of each treatment phase, each subject received a single intravenous infusion of heparin (500 U/kg body wt). Plasma was obtained from a peripheral vein and LPL was assayed in postheparin plasma and adipose tissue. Plasma postheparin LPL activity was significantly higher in the estrogen-progestin-treated subjects (2.36 +/- 0.39 micromol/ml/min) than in the placebo-treated subjects (0.46 +/- 0.22 micromol/ml/min, P less than.05). In contrast, postheparin plasma lipase activity did not differ between the placebo and estrogen-progestin-treated subjects. LPL activity in adipose tissue was significantly higher in estrogen-progestin-treated subjects (6.9 +/- 1.8 micromol/g x min) than in placebo-treated subjects (1.4 +/- 0.6 micromol/g x min, P less than.01). Plasma insulin levels were significantly higher after estrogen-progestin than after placebo treatment, but adipose tissue LPL and insulin levels did not differ between the placebo and estrogen-progestin regimens. Plasma glucose and triglyceride concentrations were significantly reduced (P less than.05) and plasma cholesterol was significantly elevated (P less than.05) by the estrogen-progestin regimen. These studies suggest that estrogen-progestin treatment of postmenopausal women decreases lipolysis in the postheparin period by altering LPL activity in adipose tissue. These findings may be one mechanism by which estrogen-progestin treatment can diminish the increase in plasma lipids that normally occurs during the postmenopausal period.Q:
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