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The efficiency of separation of adducts of guanine by 1,2-bis(9-anthryl)ethylenediamine (BAde) and 2,7-bis(9-anthryl)-9,10-dihydro-9,10-dihydroxy-1,2,7,8-tetracarboxyfluorene (BAdtetra) was evaluated by using UV-vis spectroscopy and circular dichroism, and the mechanism of the separation was assessed. By isolating the adducts, we could show that intercalated trioxalatoguanine (3Guan/tG) was a major product and, as 3Guan/tG is not stable, the hypothesis of prior ring opening was confirmed. The rate of the opening of the adducts was measured by their ability to transfer to oxidized DNA. The reduction potential of the imidazo[4,5-f][1,10]phenanthroline (IP) ligand in BAdtetra was estimated to be 0.43 V vs Ag+/Ag, and this value was used to estimate the reduction potential of the guanine radical. Quantitative amounts of the two adducts separated by this method were used to measure the reduction potential of a single adduct and that of the complex adduct. Both adducts showed a semiquinone signal at -0.1 V vs Ag+/Ag. The reduction potential of the complex was the same as that of the adduct, indicating that little electron transfer occurred

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